duoset elisa development systems kits  (R&D Systems)

Journal: Cancers

Article Title: Local Immune Changes in Early Stages of Inflammation and Carcinogenesis Correlate with the Collagen Scaffold Changes of the Colon Mucosa

doi: 10.3390/cancers13102463

Figure Lengend Snippet: Cytokine production in the full colon mucosa of DSS- or AOM-induced rats at 1 month from the end of treatments. The production of all measured cytokines in the right and left colon mucosa showed similar patterns. The significant differences were found in the IL-1α production in both left and right colon between control and treated rats. Increase of IL-6 was particularly significant in the left colon mucosa after DSS colitis induction. Decrease of TGF-β1 was relevant after AOM in all colon mucosa and significant after both treatments in the left colon mucosa, the part of the colon known to be more affected by ulcerative colitis and cancer occurrence. IFN-γ production was slightly increased after DSS (not significant), whereas it was inhibited in the right colon mucosa and especially in the left colon mucosa (not at measurable levels) after AOM pro-carcinogenic treatment. ELISA values are expressed in pg/mL. Significance by Kruskal–Wallis test: * for p < 0.05, ** for p < 0.01, and *** for p < 0.001.

Article Snippet: The following ELISA kits were used: rat IL-1α Matched Antibody Pairs kit (eBioscience, San Diego, CA, USA), rat IL-6 DuoSet ELISA Development kit (R&D Systems, Minneapolis, MN, USA), TGF-β CytoSet (Thermo Scientific, Waltham, MA, USA), rat IFN-γ ELISA Development kit (PeproTech, Rocky Hill, NJ, USA).

Techniques: Control, Enzyme-linked Immunosorbent Assay

Journal: Cancers

Article Title: Local Immune Changes in Early Stages of Inflammation and Carcinogenesis Correlate with the Collagen Scaffold Changes of the Colon Mucosa

doi: 10.3390/cancers13102463

Figure Lengend Snippet: The production of IL-1α, IL-6, TGF-β1, and IFN-γ in mesenteric lymph nodes and spleen of DSS- or AOM-induced rats at 1 month from the end of treatments. The cytokine production in MLNs and spleen was indicative of the impact of DSS and AOM on immunity at systemic level. Signs of inflammatory activation were present in MLNs, even at a non-significant level, except for IL-6 after AOM ( p < 0.05). Reduction (even though not reaching significance) of inflammatory cytokines and (at a lesser extent) TGF-β1 was observed in the spleen. IFN-γ resulted in being not detectable (n.d.) in AOM-treated groups. ELISA values are expressed in pg/mL. Significance was evaluated by Kruskal–Wallis test: * for p < 0.05.

Article Snippet: The following ELISA kits were used: rat IL-1α Matched Antibody Pairs kit (eBioscience, San Diego, CA, USA), rat IL-6 DuoSet ELISA Development kit (R&D Systems, Minneapolis, MN, USA), TGF-β CytoSet (Thermo Scientific, Waltham, MA, USA), rat IFN-γ ELISA Development kit (PeproTech, Rocky Hill, NJ, USA).

Techniques: Activation Assay, Enzyme-linked Immunosorbent Assay

Journal: Cephalalgia : an international journal of headache

Article Title: Meningeal norepinephrine produces headache behaviors in rats via actions both on dural afferents and fibroblasts.

doi: 10.1177/0333102414566861

Figure Lengend Snippet: Figure 4. Dural fibroblasts release interleukin-6 (IL-6) in response to norepinephrine (NE) stimulation. Dural fibroblast cultures were treated with NE (10 mM) or vehicle for one hour, washed, fresh media was applied and conditioned media was collected after 12 hours. Supernatants were desalted and lyo- philized into powder before being resuspended and analyzed. IL-6 content from vehicle-treated and NE-treated media was mea- sured by enzyme-linked immunosorbent assay (ELISA). Mean IL-6 levels were compared by a one-way analysis of variance (ANOVA) (F(3,19) ¼ 7.771, *p < 0.0001, n ¼ 6).

Article Snippet: A rat IL-6 DuoSet ELISA Development SYSTEM (R & D Systems) was used for detecting the interleukin-6 (IL-6) levels in the conditioned media.

Techniques: Enzyme-linked Immunosorbent Assay